abstract
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OBJECTIVES: The beta-adrenergic receptor (betaAR) genes are candidate genes for
obesity because of their roles in energy homeostasis and promotion of lipolysis
in human adipose tissue. Objective is to determine the association between
obesity and polymorphisms in genes of the beta(1)AR (ADRB1), beta(2)AR (ADRB2),
beta(3)AR (ADRB3), Gs protein alpha (GNAS1), to which all three beta-receptors
couple and the G protein beta3 subunit (GNB3), to which beta(3)ARs couple.
DESIGN: A case-control genetic association study.
SUBJECTS: A total of 643 black or white women enrolled in Women's Ischemia
Syndrome Evaluation (WISE) study.
MEASUREMENTS: Genotypes were determined by PCR with single primer extension.
Associations between genotype and body mass index (BMI), waist-to-hip ratio
(WHR), waist circumference, and obesity were made.
RESULTS: Polymorphisms in the three betaAR genes, GNAS1, and GNB3 were not
associated with BMI, WHR, waist circumference, or obesity. Linear and logistic
regression analyses found no contribution of either genotype or haplotype with
anthropometric measurements or obesity.
CONCLUSIONS: Our study suggests that among American women with suspected coronary
heart disease, polymorphisms in the betaARs and their G-protein-coupled receptors
do not contribute to increased BMI, WHR, waist circumference, or obesity. Given
that 50% of all women die from coronary heart disease, and a higher percentage
have heart disease during their lifetime, our results are likely generalizable to
many American women.