1. Development, validation and application of trace small biomolecule analytical methods for the detection of oxidatively-induced chemical alterations in DNA, DNA model compounds and proteins using high performance liquid chromatography and gas chromatography, including mass spectrometric detection techniques.
2. Utilization of GC/MS and LC/MS quantitative analytical methods for the detection and quantification of trace bioorganic compounds in biological matrices (cells, tissues, urine) as biomarkers for use in both small and large scale basic, environmental, clinical, pharmacological, and epidemiological studies utilizing Good Laboratory Practices.
3. Determine the reaction mechanisms involved in the formation of radiation-induced end-products in DNA and proteins (GC- and LC-based methods) from their free radical precursors (EPR methods) and the influence of environmental factors (i.e. hydration, radical scavengers, etc.) on these mechanisms.
4. Developing and implementing biomarker assays (radiation-induced DNA and protein end-products) as in-vivo detectors for human exposures to ionizing radiation.
5. Enzymatic repair of radiation-induced DNA lesions from mammalian cellular DNA.
6. Construction of indoor air quality databases and assessing health risks to human exposures to indoor air pollutants.
7. Determining the environmental fate and transport of industrial chemicals and radionuclides; assessing human exposures to these chemicals and associated risks.